Abstrak :
Ulkus traumatikus merupakan kondisi terjadinya kerusakan epitel yang berbatas jelas membentuk cekungan dan sering ditemukan pada rongga mulut disebabkan oleh trauma. Re-epitelisasi termasuk proses penyembuhan luka pada ulkus traumatikus, karena di dalam proses re-epitelisasi terjadi fase proliferasi yang memicu penutupan ulkus. Daun sirih merah memiliki zat aktif flavonoid yang memicu proliferasi sel fibroblas untuk re-epitelisasi. Tujuan penelitian ini adalah membuktikan efektivitas krim ekstrak daun sirih merah terhadap re-epitelisasi pada ulkus traumatikus gingiva tikus wistar. Jenis penelitian ini eksperimental laboratoris dengan rancangan penelitian post test only control group design yang terdiri dari 30 ekor tikus wistar jantan yang dibagi dalam 2 kelompok kontrol dan 3 kelompok perlakuan. Kelompok perlakuan diberi perlakuan krim ekstrak daun sirih merah dengan konsentrasi 15%, 30% dan 45 %, sedangkan kelompok kontrol negatif diberi perlakuan basis krim dan kelompok kontrol positif diberi krim triamcinolone acetonide. Penilaian re-epitelisasi diukur ketebalan epitel secara histometry dengan mikroskopi IX 71 (Olympus) dan dievaluasi dengan program Cell D (Olympus, Japan). Data dianalisis dengan uji one way ANOVA. Hasil penelitian adalah sediaan krim ekstrak daun sirih merah memiliki viskositas di atas 50 dPas, pH 7, daya lekat 3-15 menit dan daya sebar di atas 5 cm yang artinya krim layak digunakan. Nilai re-epitelisasi yang lebih besar ditunjukkan pada kelompok perlakuan pemberian ekstrak daun sirih merah konsentrasi 15%, 30% dan 45% dengan nilai re-epitelisasi tertinggi adalah krim ekstrak daun sirih merah 45%. Simpulan penelitian krim ekstrak daun sirih merah efektif terhadap re-epitelisasi pada ulkus traumatikus gingiva tikus wistar.
Kata Kunci : Ulkus traumatikus, penyembuhan luka, re-epitelisasi, krim ekstrak daun sirih merah, flavonoid
Kepustakaan : 11 (2003-2015)
Traumatic ulcer is a condition of the occurrence epithelium damage that has clear boundary and often found in the oral cavity caused by trauma. One of the healing process in traumatic ulcer is re-epithelialization. In re-epithelialization process, there is proliferative phase that triggered the closure of the ulcer. Red betel leaf has flavonoid as active substance which trigger the fibroblasts proliferation in re-epithelialization process. This study aims to prove the effectivity of the cream red betel leaf extract on re-epithelialization in gingival traumatic ulcer, invivo study in Wistar rat. This research was an experimental laboratory post test only control group design consisting of 30 male Wistar rat, which are divided into two control groups and three treatment groups. The treatment groups was given a cream of red betel leaf extract in concentration of 15%, 30%, 45%, while the negative control group treated by cream base and the positive control groups was given a triamcinolone acetonide cream. Rate of the re-epithelialization measured in histometri epithelial thickness by microscopy IX 71 (Olympus) and evaluated with Cell D (Olympus, Japan). Results were analyzed by one way ANOVA test. Results of the study is the preparation of red betel leaf extract cream had a viscosity of 50 dPas, pH 7 , adhesion 3-15 minutes and the scatter in the top 5 cm, which means the cream is worth using. The higher rate in re-epithelialization of the treatment group red betel leaf extract in concentration 15%, 30% and 45%. The highest value of re-epithelialization is cream red betel leaf extract 45%. The conclusions of this study is cream red betel leaf extract effective to re-epithelialization process in gingival traumatic ulcer of Wistar rat.
Keywords : Traumatic ulcer, wound healing, re-epithelialization, cream red betel leaf extract, flavonid
Bibliography : 37 (1995-2015)
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