Abstrak :
Penelitian ini berjudul “Efek Sitotoksik Ekstrak Etanolik Tanaman Sarang Semut (Myrmecodia pendens) melalui modulasi siklus sel raji (Human Burkitt’s Lymphoma)”. Penelitian ini bertujuan untuk membuktikan adanya efek sitotoksik ekstrak etanolik tanaman sarang semut (Myrmecodia pendans) dan menguji modulasi siklus sel raji menggunakan ekstrak etanolik tanaman sarang semut (Myrmecodia pendans) dengan berbagai dosis. Pembuatan ekstrak tanaman sarang semut, pengujian efektivitas ekstrak tanaman sarang semut, pembiakan sel raji serta pengujian dengan menggunakan metode MTT assay dilaksanakan di Laboratorium Pelatihan Pengujian Terpadu (LPPT) Unit III Universitas Gadjah Mada, Yogyakarta. Desain penelitian yang digunakan adalah eksperimental laboratoris dengan metode Bioassay. Penelitian ini menggunakan serbuk tanaman sarang semut yang diekstraksi menggunakan pelarut etanol. Konsentrasi ekstrak sarang semut yang digunakan sebagai variabel bebas yaitu 1000, 500, 250, 125, dan 62,5 μl/mg dengan variabel terikat survival sel raji (Human Burkitts Lymphoma). Uji aktivitas sitotoksik dilakukan dengan metode MTT assay, sedangkan uji siklus sel dilakukan dengan flowcytometri menggunakan pereaksi propidium Iodida (PI). Data hasil uji sitotoksik dianalisis menggunakan metode analisis probit sedangkan untuk uji modulasi siklus sel menggunakan analisis deskriptif kualitatif. Hasil penelitian menunjukkan bahwa ekstrak etanolik sarang semut bersifat sitotoksik terhadap sel raji dengan nilai IC50 sebesar 838,5 μg/mL. Ekstrak sarang semut mampu menghambat pertumbuhan sel raji melalui modulasi siklus sel dengan jumlah sel pada fase Sub G1 11,30, fase G1 46,49, fase S 13,84, fase G2-M 20,19 dan fase Hiperploidi 8.03.9
Kata Kunci : Human Burkitt’s Lymphoma, ekstrak sarang semut, sel raji
Kepustakaan : 44 ( 2000-2014)
entitled “Cytotoxic Effect of Myrmecodia pendens Plant Ethanoic Extract by Human Burkitt’s Lymphoma Cell Cycle Modulation”. This research aimed to prove the existence of cytotoxic effect of Myrmecodia pendens plant ethanoic extract and examine the Human Burkitt’s Lymphoma cell cycle modulation using several doses of Myrmecodia pendens plant ethanoic extract. Myrmecodia pendens plant extract fabrication and effectivity examination, Human Burkitt’s Lymphoma cell breeding and examination using MTT assay method were conducted in the 3rd Unit of Exercise Examination Integrated Laboratory of Gadjah Mada University, Yogyakarta. The research design used was experimental laboratory with Bioassay method. This research used Myrmecodia pendens plant pollen that was extracted using ethanol solution. The concentrations of Myrmecodia pendens plant extract used as independent variable were 1000, 500, 250, 125, and 62,5 μl/mg, and the dependent variable was Human Burritt’s Lymphoma cell survival. The examination of cytotoxic activity was conducted by MTT assay, while the cell cycle test was conducted by flowcytometry using prophydium Iodide (PI) reactor. Collected data was analyzed using probit analysis method, whereas cell cycle modulation test was described qualitatively. The results showed that the ethanoic extract of Myrmecodia pendens had cytotoxic characteristic toward Human Burritt’s Lymphoma cell by 838,5 μg/mL of IC50 value. Myrmecodia pendens extract was able to obstruct Human Burritt’s Lymphoma cell growth through cell cycle modulation which the amount of cells in Sub G1 phase, G1 phase, S phase, G2-M phase, and Hyperploid phase were 11.30, 46.49, 13.84, 20.19 and 8.03 respectively
Kata Kunci : Human Burkitt’s Lymphoma, Myrmecodia pendens plant extract Raji cell
Bibliography : 44 ( 2000-2014)
|
