Abstrak :
Jamur Ganoderma sp. isolat B1 dapat berpotensi sebagai obat antibiofilm pada penyembuhan periodontitis. Jamur ini memiliki senyawa bioaktif atau metabolit sekunder berupa flavonoid dan saponin yang dapat menghambat dan mendegradasi biofilm bakteri penyebab periodontitis, yaitu Aggregatibacter actinomycetemcomitans. Pengembangan jamur Ganoderma sp. isolat B1 sebagai salah satu obat antibiofilm di kedokteran gigi seharusnya tidak bersifat toksik dan memiliki sifat biokompatibilitas. Penelitian ini bertujuan untuk menganalisis efek sitotoksik ekstrak etanol jamur Ganoderma sp. isolat B1 terhadap human primary fibroblast. Evaluasi sitotoksik dengan metode MTT Assay dilakukan pada kultur sel fibroblas yang terdiri atas 12 kelompok. Kelompok perlakuan terdiri atas 10 variasi konsentrasi yaitu 1.000; 500; 250; 125; 62,50; 31,25; 15,63; 7,81; 3,91 dan 1,95 μg/ml. Kelompok kontrol positif berupa Chlorhexidine gluconate 0,12% dan kelompok kontrol negatif berupa kontrol sel (tanpa perlakuan). Aktivitas sitotoksik diukur berdasarkan persentase viabilitas fibroblas. Hasil yang diperoleh ditabulasikan, kemudian diuji dengan uji one way ANOVA dilanjutkan dengan uji Post Hoc berupa Honestly Significant Difference (HSD). Hasil penelitian menunjukkan bahwa viabilitas fibroblas tertinggi ditunjukkan pada konsentrasi 250 μg/ml, dengan persentase sebesar 94,39% dan viabilitas terendah pada konsentrasi 1.000 μg/ml dengan persentase 55,79%. Simpulan penelitian ini yaitu ekstrak etanol jamur Ganoderma sp. isolat B1 konsentrasi 500; 250; 125; 62,50; 31,25; 15,63; 7,81; 3,91 dan 1,95 μg/ml tidak bersifat toksik, sedangkan pada konsentrasi 1.000 μg/ml bersifat toksik terhadap fibroblas.
Kata Kunci : Sitotoksik, jamur Ganoderma sp. isolat B1, viabilitas, fibroblas.
Kepustakaan : 80 (1971-2016)
Ganoderma sp. isolate B1 has a promising potential as anti-biofilm in the healing process of periodontitis. This type of fungi has bioactive substances or secondary metabolites known as flavonoid and saponin that can inhibit and degrade bio-film of Aggregatibacter actinomycetemcomitans, periodontitis-causing bacteria. The development of Ganoderma sp. isolate B1 as one of anti-biofilm drugs in dental medicine should be done in non-toxic and biocompatible fashion. This study was conducted to analyze the cytotoxic effect of ethanol extract of Ganoderma sp. isolate B1 to human primary fibroblast. The cytotoxic activity was evaluated using MTT Assay conducted in 12 groups of fibroblast cells culture. The experiment group consisted of 10 different concentrations which were 1,000; 500; 250; 125; 62.50; 31.25; 15.63; 7.81; 3.91 and 1.95 μg/ml. The positive control group was Chlorhexidine gluconate 0.12% while the negative control group was control cell (without intervention). The cytotoxic activity was measured according to the percent of viability of the fibroblast. The results obtained were tabulated and examined using one way ANOVA test followed by Post Hoc test which was Honestly Significant Difference (HSD). The results showed that the highest fibroblast viability was found in 250 μg/ml group with the percent of viability of 94.39% and the lowest viability was found in 1.000 μg/ml group with the percent of viability of 55.79%. This study concluded that ethanol extract of Ganoderma sp. isolate B1 was found to be non-toxic to human primary fibroblast in the concentration of 500; 250; 125; 62.50; 31.25; 15.63; 7.81; 3.91 and 1.95 μg/ml, while in the concentration of 1,000 μg/ml, it appeared to be significantly toxic.
Keywords : Cytotoxic, Ganoderma sp. isolate B1 fungi, viability, fibroblast.
Reference : 80 (1971-2016)
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