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Abstrak :
Vanda tricolor Lindl. orchid is widespread in Java, Bali, and Sulawesi, V. tricolor on its natural habitat became scarce due to forest destruction and natural disasters and human activities. To overcome this, it can be done by the propagation of orchids using in vitro culture. In vitro culture is an improvement effort to prevent extinction. This technique can provide new natural orchids quickly with good quality. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates is expected to increase the growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so that there are 18 trial units. The source of the explants used was the orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The treatment with a significant effect was followed by the Least Significant Difference test (LSD) at an error rate of 95%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.
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